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M-CSF/CSF1 ELISA 试剂盒

CSF1 适用: 小鼠 Colorimetric Sandwich ELISA 1 pg/mL - 350 pg/mL Cell Lysate, Tissue Lysate
产品编号 ABIN6951452
发货至: 中国
  • 抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    适用
    • 11
    • 6
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1 pg/mL - 350 pg/mL
    最低检测浓度
    1 pg/mL
    应用范围
    ELISA
    原理
    Mouse M-CSF ELISA Kit for cell and tissue lysate samples.
    样品类型
    Cell Lysate, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CSF1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    别名
    M-CSF (CSF1 产品)
    别名
    CSF-1 ELISA Kit, MCSF ELISA Kit, C87615 ELISA Kit, Csfm ELISA Kit, op ELISA Kit, csf1-1 ELISA Kit, zgc:172186 ELISA Kit, CSF1 ELISA Kit, csf1-2 ELISA Kit, zgc:158436 ELISA Kit, colony stimulating factor 1 ELISA Kit, colony stimulating factor 1 (macrophage) ELISA Kit, colony stimulating factor 1a (macrophage) ELISA Kit, macrophage colony-stimulating factor 1 ELISA Kit, macrophage colony stimulating factor ELISA Kit, colony stimulating factor 1b (macrophage) ELISA Kit, CSF1 ELISA Kit, Csf1 ELISA Kit, csf1a ELISA Kit, LOC396599 ELISA Kit, LOC100860895 ELISA Kit, csf1b ELISA Kit
    基因ID
    12977
    UniProt
    P07141
    途径
    RTK signaling
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