HRAS ELISA 试剂盒
HRAS
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all HRAS ELISA试剂盒
- HRAS (HRas proto-oncogene, GTPase (HRAS))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human HRAS ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling HRAS ELISA Kit
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- Discover our top product HRAS ELISA Kit
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HRas proto-oncogene, GTPase (HRAS) ELISA Kit
HRAS 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all HRAS ELISA试剂盒
- HRAS (HRas proto-oncogene, GTPase (HRAS))
- 别名
- HRAS (HRAS 产品)
- 别名
- C-BAS/HAS ELISA Kit, C-H-RAS ELISA Kit, C-HA-RAS1 ELISA Kit, CTLO ELISA Kit, H-RASIDX ELISA Kit, HAMSV ELISA Kit, HRAS1 ELISA Kit, K-RAS ELISA Kit, N-RAS ELISA Kit, RASH1 ELISA Kit, hras ELISA Kit, zgc:110250 ELISA Kit, HRAS ELISA Kit, H-RAS ELISA Kit, c-H-ras ELISA Kit, H-Ras ELISA Kit, K-Ras ELISA Kit, hras1 ELISA Kit, rash1 ELISA Kit, ras ELISA Kit, N-Ras ELISA Kit, c-bas/has ELISA Kit, H-ras ELISA Kit, Ha-ras ELISA Kit, Harvey-ras ELISA Kit, Hras-1 ELISA Kit, Kras2 ELISA Kit, c-Ha-ras ELISA Kit, c-rasHa ELISA Kit, hrasl ELISA Kit, zgc:110734 ELISA Kit, HRas proto-oncogene, GTPase ELISA Kit, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a ELISA Kit, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene ELISA Kit, Harvey rat sarcoma viral oncogene homolog L homeolog ELISA Kit, Harvey rat sarcoma viral oncogene homolog ELISA Kit, Harvey rat sarcoma virus oncogene ELISA Kit, NRAS proto-oncogene, GTPase ELISA Kit, -Ha-ras Harvey rat sarcoma viral oncogene homolog b ELISA Kit, HRAS ELISA Kit, hrasa ELISA Kit, LOC733587 ELISA Kit, Hras ELISA Kit, hras.L ELISA Kit, hras ELISA Kit, NRAS ELISA Kit, hrasb ELISA Kit
- 基因ID
- 3265
- UniProt
- P01112
- 途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling
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