GADD45A ELISA 试剂盒
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- 抗原 See all GADD45A ELISA试剂盒
- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GADD45A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product GADD45A ELISA Kit
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Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA Kit
GADD45A 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA KitGADD45A 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) ELISA KitGADD45A 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all GADD45A ELISA试剂盒
- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
- 别名
- GADD45A (GADD45A 产品)
- 别名
- MGC53491 ELISA Kit, zgc:91795 ELISA Kit, gadd45a ELISA Kit, GADD45A ELISA Kit, ddit1 ELISA Kit, gadd45 ELISA Kit, GADD45 ELISA Kit, AA545191 ELISA Kit, Ddit1 ELISA Kit, DDIT1 ELISA Kit, Gadd45 ELISA Kit, growth arrest and DNA damage inducible alpha L homeolog ELISA Kit, growth arrest and DNA-damage-inducible, alpha, b ELISA Kit, growth arrest and DNA damage inducible alpha ELISA Kit, growth arrest and DNA damage-inducible protein 45 ELISA Kit, growth arrest and DNA-damage-inducible 45 alpha ELISA Kit, growth arrest and DNA-damage-inducible, alpha ELISA Kit, gadd45a.L ELISA Kit, gadd45ab ELISA Kit, GADD45A ELISA Kit, gadd45a ELISA Kit, GADD45 ELISA Kit, Gadd45a ELISA Kit
- 基因ID
- 1647
- UniProt
- P24522
- 途径
- p53 Pathway, Cell Division Cycle
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