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PPIL2 ELISA 试剂盒

PPIL2 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951429
发货至: 中国
  • 抗原 See all PPIL2 products
    PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human PPIL2 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all PPIL2 products
    PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
    别名
    PPIL2 (PPIL2 产品)
    别名
    cyc4 ELISA Kit, cyp60 ELISA Kit, hcyp-60 ELISA Kit, CYC4 ELISA Kit, CYP60 ELISA Kit, Cyp-60 ELISA Kit, UBOX7 ELISA Kit, hCyP-60 ELISA Kit, 0610009L05Rik ELISA Kit, 1700016N17Rik ELISA Kit, 4921520K19Rik ELISA Kit, 4930511F14Rik ELISA Kit, AA589416 ELISA Kit, C130078A06Rik ELISA Kit, si:dkeyp-86g2.1 ELISA Kit, zgc:56616 ELISA Kit, zgc:86735 ELISA Kit, peptidylprolyl isomerase like 2 S homeolog ELISA Kit, peptidylprolyl isomerase like 2 ELISA Kit, peptidylprolyl isomerase (cyclophilin)-like 2 ELISA Kit, ppil2.S ELISA Kit, PPIL2 ELISA Kit, ppil2 ELISA Kit, Ppil2 ELISA Kit
    基因ID
    23759
    UniProt
    Q13356
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