CDKN2D ELISA 试剂盒
CDKN2D
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
抗原
See all CDKN2D products
-
CDKN2D
(Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
-
适用
-
人
-
检测方法
-
Colorimetric
-
实验类型
-
Sandwich ELISA
-
应用范围
-
ELISA
-
原理
-
Human CDKN2D ELISA Kit.
-
样品类型
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
应用备注
-
Optimal working dilution should be determined by the investigator.
-
板类型
-
Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
-
仅限研究用
-
-
有效期
-
6 months
-
-
抗原
See all CDKN2D products
-
CDKN2D
(Cyclin-Dependent Kinase Inhibitor 2D (p19, Inhibits CDK4) (CDKN2D))
-
别名
-
CDKN2D (CDKN2D 产品)
-
别名
-
ink4d ELISA Kit, p19 ELISA Kit, p19-ink4d ELISA Kit, CDKN2D ELISA Kit, ank2 ELISA Kit, INK4D ELISA Kit, p19-INK4D ELISA Kit, INK4d ELISA Kit, p19INK4d ELISA Kit, cyclin-dependent kinase inhibitor 2D S homeolog ELISA Kit, cyclin dependent kinase inhibitor 2D ELISA Kit, cyclin-dependent kinase inhibitor 2D ELISA Kit, Cyclin-dependent kinase 4 inhibitor D ELISA Kit, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) ELISA Kit, similar to cyclin-dependent kinase inhibitor 2D ELISA Kit, cdkn2d.S ELISA Kit, CDKN2D ELISA Kit, cdkn2d ELISA Kit, cdn2d ELISA Kit, Cdkn2d ELISA Kit
-
基因ID
-
1032
-
UniProt
-
P55273
-
途径
-
Cell Division Cycle, Sensory Perception of Sound, Mitotic G1-G1/S Phases, Negative Regulation of intrinsic apoptotic Signaling
-