CRIP2 ELISA 试剂盒
CRIP2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CRIP2 products
- CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CRIP2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all CRIP2 products
- CRIP2 (Cysteine-Rich Protein 2 (CRIP2))
- 别名
- CRIP2 (CRIP2 产品)
- 别名
- CRIP ELISA Kit, CRP2 ELISA Kit, ESP1 ELISA Kit, C6orf210 ELISA Kit, COQ10D3 ELISA Kit, DLP1 ELISA Kit, bA59I9.3 ELISA Kit, hDLP1 ELISA Kit, AW551867 ELISA Kit, Crp2 ELISA Kit, SmLim ELISA Kit, SmLIM ELISA Kit, zgc:77740 ELISA Kit, 0610010I23Rik ELISA Kit, AW743261 ELISA Kit, C77570 ELISA Kit, CRP4 ELISA Kit, Crp ELISA Kit, Hlp ELISA Kit, wu:fb30h10 ELISA Kit, zgc:55408 ELISA Kit, cysteine rich protein 2 ELISA Kit, decaprenyl diphosphate synthase subunit 2 ELISA Kit, cysteine and glycine-rich protein 2 ELISA Kit, cysteine and glycine rich protein 2 ELISA Kit, cysteine-rich protein 2 ELISA Kit, CRIP2 ELISA Kit, PDSS2 ELISA Kit, Csrp2 ELISA Kit, csrp2 ELISA Kit, CSRP2 ELISA Kit, Crip2 ELISA Kit, crip2 ELISA Kit
- 基因ID
- 1397
- UniProt
- P52943
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