CD45 ELISA 试剂盒
PTPRC
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all CD45 (PTPRC) ELISA试剂盒
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CD45 (PTPRC)
(Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
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人
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Human PTPRC ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注
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Optimal working dilution should be determined by the investigator.
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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有效期
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6 months
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抗原
See all CD45 (PTPRC) ELISA试剂盒
-
CD45 (PTPRC)
(Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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别名
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PTPRC (PTPRC 产品)
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别名
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PTPRC ELISA Kit, si:dkeyp-47c5.1 ELISA Kit, B220 ELISA Kit, CD45 ELISA Kit, CD45R ELISA Kit, GP180 ELISA Kit, L-CA ELISA Kit, LCA ELISA Kit, LY5 ELISA Kit, T200 ELISA Kit, Cd45 ELISA Kit, Ly-5 ELISA Kit, Lyt-4 ELISA Kit, loc ELISA Kit, Lca ELISA Kit, RT7 ELISA Kit, cd45 ELISA Kit, protein tyrosine phosphatase, receptor type C ELISA Kit, protein tyrosine phosphatase, receptor type, C ELISA Kit, receptor-type tyrosine-protein phosphatase C ELISA Kit, PTPRC ELISA Kit, ptprc ELISA Kit, LOC100074271 ELISA Kit, Ptprc ELISA Kit
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基因ID
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5788
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UniProt
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P08575
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途径
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TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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