BNIP3L/NIX ELISA 试剂盒
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- 抗原 See all BNIP3L/NIX (BNIP3L) ELISA试剂盒
- BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human BNIP3L ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling BNIP3L ELISA Kit
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- Discover our top product BNIP3L ELISA Kit
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BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L) ELISA Kit
BNIP3L 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L) ELISA KitBNIP3L 适用: 人 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all BNIP3L/NIX (BNIP3L) ELISA试剂盒
- BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))
- 别名
- BNIP3L (BNIP3L 产品)
- 别名
- BNIP3a ELISA Kit, NIX ELISA Kit, NIP3 ELISA Kit, Nix ELISA Kit, Nip3 ELISA Kit, C86132 ELISA Kit, D14Ertd719e ELISA Kit, Nip3L ELISA Kit, bnip3l ELISA Kit, nip3 ELISA Kit, zgc:110478 ELISA Kit, bnip3l2 ELISA Kit, wu:fb52b03 ELISA Kit, wu:fc02h04 ELISA Kit, BCL2 interacting protein 3 like ELISA Kit, BCL2 interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3 ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like b ELISA Kit, BCL2/adenovirus E1B interacting protein 3-like a ELISA Kit, BNIP3L ELISA Kit, BNIP3 ELISA Kit, Bnip3l ELISA Kit, Bnip3 ELISA Kit, bnip3lb ELISA Kit, bnip3la ELISA Kit
- 基因ID
- 665
- UniProt
- O60238
- 途径
- Autophagy
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