SNAP25 ELISA 试剂盒
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- 抗原 See all SNAP25 ELISA试剂盒
- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SNAP25 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all SNAP25 ELISA试剂盒
- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
- 别名
- SNAP25 (SNAP25 产品)
- 别名
- RIC-4 ELISA Kit, RIC4 ELISA Kit, SEC9 ELISA Kit, SNAP ELISA Kit, SNAP-25 ELISA Kit, bA416N4.2 ELISA Kit, dJ1068F16.2 ELISA Kit, SNAP-25B ELISA Kit, SNAP-25a ELISA Kit, ric-4 ELISA Kit, ric4 ELISA Kit, sec9 ELISA Kit, snap-25 ELISA Kit, Bdr ELISA Kit, sp ELISA Kit, fj55h11 ELISA Kit, snap25 ELISA Kit, wu:fj55h11 ELISA Kit, Snap ELISA Kit, snap25.2 ELISA Kit, wu:fj33h05 ELISA Kit, synaptosome associated protein 25 ELISA Kit, synaptosome associated protein 25kDa S homeolog ELISA Kit, synaptosomal-associated protein, 25kDa ELISA Kit, synaptosomal-associated protein 25 ELISA Kit, synaptosome associated protein 25kDa ELISA Kit, synaptosome associated protein 25kDa L homeolog ELISA Kit, zgc:101731 ELISA Kit, synaptosomal-associated protein, 25b ELISA Kit, SNAP25 ELISA Kit, Snap25 ELISA Kit, snap25.S ELISA Kit, snap25 ELISA Kit, BACOIKO003_07 ELISA Kit, Tsp_10811 ELISA Kit, snap25.L ELISA Kit, zgc:101731 ELISA Kit, snap25b ELISA Kit
- 基因ID
- 6616
- UniProt
- P60880
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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