NT5C3 ELISA 试剂盒
NT5C3
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all NT5C3 products
- NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human NT5C3A ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all NT5C3 products
- NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))
- 别名
- NT5C3A (NT5C3 产品)
- 别名
- 1600024P05Rik ELISA Kit, 2610206B05Rik ELISA Kit, 3110004A18Rik ELISA Kit, AI842776 ELISA Kit, Nt5c3a ELISA Kit, Nt5y ELISA Kit, PN-1 ELISA Kit, PN-I ELISA Kit, PSN1 ELISA Kit, UMPH ELISA Kit, Umph-1 ELISA Kit, Umph1 ELISA Kit, cN-III ELISA Kit, lupin ELISA Kit, p36 ELISA Kit, fb30h07 ELISA Kit, nt5c3 ELISA Kit, wu:fb30h07 ELISA Kit, zgc:66117 ELISA Kit, NT5C3 ELISA Kit, PYN1 ELISA Kit, P5'N-1 ELISA Kit, P5N-1 ELISA Kit, POMP ELISA Kit, UMPH1 ELISA Kit, hUMP1 ELISA Kit, Nt5c3 ELISA Kit, 5'-nucleotidase, cytosolic III ELISA Kit, 5'-nucleotidase, cytosolic IIIA ELISA Kit, cytosolic 5'-nucleotidase 3A ELISA Kit, Nt5c3 ELISA Kit, nt5c3a ELISA Kit, NT5C3A ELISA Kit, LOC534181 ELISA Kit, Nt5c3a ELISA Kit, nt5c3a.L ELISA Kit, Bm1_56095 ELISA Kit
- 基因ID
- 51251
- UniProt
- Q9H0P0
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