ATP5F1D ELISA 试剂盒
ATP5F1D
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ATP5F1D ELISA试剂盒
- ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ATP5D ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all ATP5F1D ELISA试剂盒
- ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))
- 别名
- ATP5D (ATP5F1D 产品)
- 别名
- fk58f09 ELISA Kit, zgc:73303 ELISA Kit, wu:fk58f09 ELISA Kit, MGC85306 ELISA Kit, GB17255 ELISA Kit, DDBDRAFT_0216607 ELISA Kit, DDBDRAFT_0238330 ELISA Kit, DDB_0216607 ELISA Kit, DDB_0238330 ELISA Kit, 0610008F14Rik ELISA Kit, 1500000I11Rik ELISA Kit, AA960090 ELISA Kit, AI876556 ELISA Kit, AU020773 ELISA Kit, C85518 ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit ELISA Kit, ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit L homeolog ELISA Kit, ATP synthase F1 delta ELISA Kit, atp5d ELISA Kit, atp5d.L ELISA Kit, ATP5D ELISA Kit, LOC552682 ELISA Kit, atp5D ELISA Kit, Atp5d ELISA Kit
- 基因ID
- 513
- UniProt
- P30049
- 途径
- Proton Transport, Ribonucleoside Biosynthetic Process
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