CD300LG ELISA 试剂盒
TREM4
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CD300LG (TREM4) ELISA试剂盒
- CD300LG (TREM4) (Triggering Receptor Expressed On Myeloid Cells 4 (TREM4))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat CD300LG ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TREM4 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all CD300LG (TREM4) ELISA试剂盒
- CD300LG (TREM4) (Triggering Receptor Expressed On Myeloid Cells 4 (TREM4))
- 别名
- CD300LG (TREM4 产品)
- 别名
- RGD1311074 ELISA Kit, 2310016B05Rik ELISA Kit, Clm9 ELISA Kit, D11Ertd736e ELISA Kit, CLM-9 ELISA Kit, CLM9 ELISA Kit, NEPMUCIN ELISA Kit, TREM-4 ELISA Kit, TREM4 ELISA Kit, Cd300 molecule-like family member G ELISA Kit, CD300 molecule like family member G ELISA Kit, CD300 molecule like family member g ELISA Kit, triggering receptor expressed on myeloid cells 4 ELISA Kit, Cd300lg ELISA Kit, CD300LG ELISA Kit, Trem4 ELISA Kit
- UniProt
- M0R459
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