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ACAA1 ELISA 试剂盒

ACAA1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951298
发货至: 中国
  • 抗原 See all ACAA1 products
    ACAA1 (Acetyl-CoA Acyltransferase 1 (ACAA1))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human ACAA1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all ACAA1 products
    ACAA1 (Acetyl-CoA Acyltransferase 1 (ACAA1))
    别名
    ACAA1 (ACAA1 产品)
    别名
    ACAA ELISA Kit, PTHIO ELISA Kit, THIO ELISA Kit, Acaa ELISA Kit, Acaa1 ELISA Kit, D9Ertd25e ELISA Kit, PTL ELISA Kit, zgc:92385 ELISA Kit, acaa ELISA Kit, pthio ELISA Kit, thio ELISA Kit, A2MP ELISA Kit, Pktaa ELISA Kit, acetyl-CoA acyltransferase 1 ELISA Kit, acetyl-Coenzyme A acyltransferase 1A ELISA Kit, acetyl-CoA acyltransferase 1 L homeolog ELISA Kit, ACP synthase ELISA Kit, alpha-2-macroglobulin pseudogene 1 ELISA Kit, acetyl-CoA acyltransferase 1A ELISA Kit, ACAA1 ELISA Kit, Acaa1a ELISA Kit, acaa1.L ELISA Kit, acaa1 ELISA Kit, AF_RS00150 ELISA Kit, A2MP1 ELISA Kit, Acaa1 ELISA Kit
    基因ID
    30
    UniProt
    P09110
    途径
    Monocarboxylic Acid Catabolic Process
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