HYOU1 ELISA 试剂盒
-
- 抗原 See all HYOU1 ELISA试剂盒
- HYOU1 (Hypoxia Up Regulated 1 (HYOU1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human HYOU1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling HYOU1 ELISA Kit
- Top Product
- Discover our top product HYOU1 ELISA Kit
-
Hypoxia Up Regulated 1 (HYOU1) ELISA Kit
HYOU1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hypoxia Up Regulated 1 (HYOU1) ELISA KitHYOU1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all HYOU1 ELISA试剂盒
- HYOU1 (Hypoxia Up Regulated 1 (HYOU1))
- 别名
- HYOU1 (HYOU1 产品)
- 别名
- GRP-170 ELISA Kit, Grp170 ELISA Kit, HSP12A ELISA Kit, ORP-150 ELISA Kit, ORP150 ELISA Kit, AI415631 ELISA Kit, CBP-140 ELISA Kit, Cab140 ELISA Kit, Orp150 ELISA Kit, id:ibd5056 ELISA Kit, wu:fd14c05 ELISA Kit, zgc:56019 ELISA Kit, HYOU1 ELISA Kit, orp150 ELISA Kit, GRP170 ELISA Kit, hypoxia up-regulated 1 ELISA Kit, hypoxia up-regulated protein 1 ELISA Kit, hypoxia up-regulated 1 S homeolog ELISA Kit, HYOU1 ELISA Kit, Hyou1 ELISA Kit, hyou1 ELISA Kit, LOC100380749 ELISA Kit, hyou1.S ELISA Kit
- 基因ID
- 10525
- UniProt
- Q9Y4L1
- 途径
- ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome
-
