Syntaxin 7 ELISA 试剂盒
STX7
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Syntaxin 7 (STX7) products
- Syntaxin 7 (STX7)
- 适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat STX7 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all Syntaxin 7 (STX7) products
- Syntaxin 7 (STX7)
- 别名
- STX7 (STX7 产品)
- 别名
- Aval ELISA Kit, Avl ELISA Kit, CG5081 ELISA Kit, Dm Syx7 ELISA Kit, DmSyx7 ELISA Kit, Dmel\\CG5081 ELISA Kit, FCP-C ELISA Kit, SYX7 ELISA Kit, Syx ELISA Kit, avl ELISA Kit, dSyn7 ELISA Kit, syx7 ELISA Kit, syx7/avl ELISA Kit, syntaxin-7 ELISA Kit, F27G20.2 ELISA Kit, SYNTAXIN 24 ELISA Kit, SYP24 ELISA Kit, DDBDRAFT_0187598 ELISA Kit, DDBDRAFT_0231537 ELISA Kit, DDB_0187598 ELISA Kit, DDB_0231537 ELISA Kit, Syntaxin-7 ELISA Kit, STX7 ELISA Kit, AI315064 ELISA Kit, AI317144 ELISA Kit, AW107239 ELISA Kit, Syn7 ELISA Kit, Syntaxin 7 ELISA Kit, syntaxin 7 ELISA Kit, syntaxin ELISA Kit, Syx7 ELISA Kit, STX7 ELISA Kit, ATSYP24 ELISA Kit, syn7A ELISA Kit, stx7 ELISA Kit, Stx7 ELISA Kit
- 基因ID
- 60466
- UniProt
- O70257
- 途径
- Synaptic Vesicle Exocytosis
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