SOCS6 ELISA 试剂盒
SOCS6
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SOCS6 ELISA试剂盒
- SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human socs6 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all SOCS6 ELISA试剂盒
- SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))
- 别名
- socs6 (SOCS6 产品)
- 别名
- CIS-4 ELISA Kit, CIS4 ELISA Kit, HSPC060 ELISA Kit, SOCS-4 ELISA Kit, SOCS-6 ELISA Kit, SOCS4 ELISA Kit, SSI4 ELISA Kit, STAI4 ELISA Kit, STATI4 ELISA Kit, socs6 ELISA Kit, MGC81477 ELISA Kit, SOCS6 ELISA Kit, 1500012M23Rik ELISA Kit, 5830401B18Rik ELISA Kit, AI447482 ELISA Kit, Cis4 ELISA Kit, Cish4 ELISA Kit, Socs4 ELISA Kit, socs6l ELISA Kit, zgc:64107 ELISA Kit, wu:fb77f06 ELISA Kit, suppressor of cytokine signaling 6 ELISA Kit, suppressor of cytokine signaling 6 L homeolog ELISA Kit, suppressor of cytokine signaling ELISA Kit, suppressor of cytokine signaling 6b ELISA Kit, SOCS6 ELISA Kit, Socs6 ELISA Kit, socs6.L ELISA Kit, socs6 ELISA Kit, socs6b ELISA Kit
- 基因ID
- 9306
- UniProt
- O14544
- 途径
- JAK/STAT Signaling, Carbohydrate Homeostasis
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