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Ribokinase ELISA 试剂盒

RBKS 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951225
发货至: 中国
  • 抗原 See all Ribokinase (RBKS) ELISA试剂盒
    Ribokinase (RBKS)
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human RBKS ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Ribokinase (RBKS) ELISA试剂盒
    Ribokinase (RBKS)
    别名
    RBKS (RBKS 产品)
    别名
    RBSK ELISA Kit, 5230400M11Rik ELISA Kit, AI326806 ELISA Kit, zgc:86813 ELISA Kit, MGC131284 ELISA Kit, F20D23.14 ELISA Kit, F20D23_14 ELISA Kit, 20.t00015 ELISA Kit, ribokinase ELISA Kit, Ribokinase ELISA Kit, ribokinase L homeolog ELISA Kit, pfkB-like carbohydrate kinase family protein ELISA Kit, RBKS ELISA Kit, Rbks ELISA Kit, F07A11.5 ELISA Kit, rbks ELISA Kit, rbks.L ELISA Kit, AT1G17160 ELISA Kit, EHI_138020 ELISA Kit, Tb11.03.0090 ELISA Kit, Mrub_0200 ELISA Kit, rbkS ELISA Kit, Ndas_4158 ELISA Kit, Mesil_0220 ELISA Kit, Arch_1729 ELISA Kit, Trad_0706 ELISA Kit, Halhy_5191 ELISA Kit, Mesop_4859 ELISA Kit, Theth_0467 ELISA Kit
    基因ID
    64080
    UniProt
    Q9H477
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