Ribokinase ELISA 试剂盒
RBKS
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Ribokinase (RBKS) ELISA试剂盒
- Ribokinase (RBKS)
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human RBKS ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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-
Ribokinase (RBKS) ELISA Kit
RBKS 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all Ribokinase (RBKS) ELISA试剂盒
- Ribokinase (RBKS)
- 别名
- RBKS (RBKS 产品)
- 别名
- RBSK ELISA Kit, 5230400M11Rik ELISA Kit, AI326806 ELISA Kit, zgc:86813 ELISA Kit, MGC131284 ELISA Kit, F20D23.14 ELISA Kit, F20D23_14 ELISA Kit, 20.t00015 ELISA Kit, ribokinase ELISA Kit, Ribokinase ELISA Kit, ribokinase L homeolog ELISA Kit, pfkB-like carbohydrate kinase family protein ELISA Kit, RBKS ELISA Kit, Rbks ELISA Kit, F07A11.5 ELISA Kit, rbks ELISA Kit, rbks.L ELISA Kit, AT1G17160 ELISA Kit, EHI_138020 ELISA Kit, Tb11.03.0090 ELISA Kit, Mrub_0200 ELISA Kit, rbkS ELISA Kit, Ndas_4158 ELISA Kit, Mesil_0220 ELISA Kit, Arch_1729 ELISA Kit, Trad_0706 ELISA Kit, Halhy_5191 ELISA Kit, Mesop_4859 ELISA Kit, Theth_0467 ELISA Kit
- 基因ID
- 64080
- UniProt
- Q9H477
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