RAB7A ELISA 试剂盒
RAB7A
适用: 大鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all RAB7A ELISA试剂盒
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RAB7A
(RAB7A, Member RAS Oncogene Family (RAB7A))
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适用
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大鼠
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Rat RAB7A ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product RAB7A ELISA Kit
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应用备注
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Optimal working dilution should be determined by the investigator.
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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有效期
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6 months
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抗原
See all RAB7A ELISA试剂盒
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RAB7A
(RAB7A, Member RAS Oncogene Family (RAB7A))
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别名
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RAB7A (RAB7A 产品)
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别名
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RAB5 ELISA Kit, PRO2706 ELISA Kit, RAB7 ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit, pro2706 ELISA Kit, RAB7A ELISA Kit, LOC100231734 ELISA Kit, ARABIDOPSIS RAB GTPASE HOMOLOG G2 ELISA Kit, ATRAB7A ELISA Kit, ATRABG2 ELISA Kit, F7D8.20 ELISA Kit, F7D8_20 ELISA Kit, RAB GTPase homolog 7A ELISA Kit, DDBDRAFT_0190210 ELISA Kit, DDBDRAFT_0191507 ELISA Kit, DDB_0190210 ELISA Kit, DDB_0191507 ELISA Kit, Rab7a ELISA Kit, rab-7 ELISA Kit, zgc:64058 ELISA Kit, RAB5A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family L homeolog ELISA Kit, rab7A protein ELISA Kit, RAB GTPase homolog 7A ELISA Kit, Rab GTPase ELISA Kit, ras-related protein rab-7a ELISA Kit, RAB7, member RAS oncogene family ELISA Kit, RAB5A ELISA Kit, RAB7A ELISA Kit, Rab7a ELISA Kit, rab7a.L ELISA Kit, rab7a ELISA Kit, rab7A ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit
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基因ID
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29448
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UniProt
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P09527
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途径
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EGFR Signaling Pathway, Maintenance of Protein Location, SARS-CoV-2 Protein Interactome
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