PVALB ELISA 试剂盒
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- 抗原 See all PVALB ELISA试剂盒
- PVALB (Parvalbumin (PVALB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PVALB ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PVALB ELISA Kit
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Parvalbumin (PVALB) ELISA Kit
PVALB 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Parvalbumin (PVALB) ELISA KitPVALB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Parvalbumin (PVALB) ELISA KitPVALB 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all PVALB ELISA试剂盒
- PVALB (Parvalbumin (PVALB))
- 别名
- PVALB (PVALB 产品)
- 别名
- D22S749 ELISA Kit, PV ELISA Kit, Parv ELISA Kit, Pva ELISA Kit, PALB1 ELISA Kit, pvalb1 ELISA Kit, MGC107903 ELISA Kit, parvalbumin ELISA Kit, parvalbumin, gene 1 ELISA Kit, parvalbumin, gene 1 S homeolog ELISA Kit, PVALB ELISA Kit, Pvalb ELISA Kit, pvalb.1 ELISA Kit, pvalb.1.S ELISA Kit
- 基因ID
- 5816
- UniProt
- P20472
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