PMVK ELISA 试剂盒
PMVK
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PMVK ELISA试剂盒
- PMVK (Phosphomevalonate Kinase (PMVK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PMVK ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PMVK ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all PMVK ELISA试剂盒
- PMVK (Phosphomevalonate Kinase (PMVK))
- 别名
- PMVK (PMVK 产品)
- 别名
- MPK_Bm ELISA Kit, F5M6.9 ELISA Kit, F5M6_9 ELISA Kit, zgc:162701 ELISA Kit, HUMPMKI ELISA Kit, PMK ELISA Kit, PMKA ELISA Kit, PMKASE ELISA Kit, 1110011E12Rik ELISA Kit, 2900002L22Rik ELISA Kit, AV001327 ELISA Kit, phosphomevalonate kinase ELISA Kit, GHMP kinase family protein ELISA Kit, PMVK ELISA Kit, Mpk ELISA Kit, AT1G31910 ELISA Kit, CpipJ_CPIJ015413 ELISA Kit, Bm1_05275 ELISA Kit, pmvk ELISA Kit, LOAG_11811 ELISA Kit, Tsp_06629 ELISA Kit, LOC100282968 ELISA Kit, Pmvk ELISA Kit
- 基因ID
- 10654
- UniProt
- Q15126
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