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Nodal ELISA 试剂盒

NODAL 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951187
发货至: 中国
  • 抗原 See all Nodal (NODAL) ELISA试剂盒
    Nodal (NODAL) (Nodal Homolog (NODAL))
    适用
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Nodal ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Nodal (NODAL) ELISA试剂盒
    Nodal (NODAL) (Nodal Homolog (NODAL))
    别名
    Nodal (NODAL 产品)
    别名
    NODAL ELISA Kit, Tg.413d ELISA Kit, HTX5 ELISA Kit, nodal4 ELISA Kit, nodal4-A ELISA Kit, nr4 ELISA Kit, xnr4 ELISA Kit, Xnr-1 ELISA Kit, nodal ELISA Kit, nr1 ELISA Kit, nr1-A ELISA Kit, nodal growth differentiation factor ELISA Kit, nodal homolog (mouse) ELISA Kit, nodal ELISA Kit, nodal growth differentiation factor L homeolog ELISA Kit, nodal homolog 1 L homeolog ELISA Kit, Nodal ELISA Kit, NODAL ELISA Kit, nodal.L ELISA Kit, nodal1.L ELISA Kit
    基因ID
    4838
    UniProt
    Q96S42
    途径
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity
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