MCL-1 ELISA 试剂盒
MCL1
适用: Cow
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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抗原
See all MCL-1 (MCL1) ELISA试剂盒
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MCL-1 (MCL1)
(Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
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适用
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Cow
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检测方法
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Colorimetric
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实验类型
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Sandwich ELISA
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应用范围
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ELISA
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原理
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Bovine MCL1 ELISA Kit.
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样品类型
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product MCL1 ELISA Kit
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应用备注
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Optimal working dilution should be determined by the investigator.
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板类型
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Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
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仅限研究用
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有效期
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6 months
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抗原
See all MCL-1 (MCL1) ELISA试剂盒
-
MCL-1 (MCL1)
(Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
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别名
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MCL1 (MCL1 产品)
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别名
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BCL2L3 ELISA Kit, EAT ELISA Kit, MCL1-ES ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, Mcl-1 ELISA Kit, TM ELISA Kit, bcl2-L-3 ELISA Kit, mcl1/EAT ELISA Kit, AW556805 ELISA Kit, mcl-1 ELISA Kit, fb72f02 ELISA Kit, wu:fb70a08 ELISA Kit, wu:fb72f02 ELISA Kit, zfMLP1 ELISA Kit, zfMcl-1a ELISA Kit, zgc:109835 ELISA Kit, zgc:109763 ELISA Kit, E3 ELISA Kit, E3-2 ELISA Kit, RNE3 ELISA Kit, RNU107 ELISA Kit, SNORA63A ELISA Kit, elF-4AII ELISA Kit, MCL1, BCL2 family apoptosis regulator ELISA Kit, myeloid cell leukemia sequence 1 ELISA Kit, MCL1, BCL2 family apoptosis regulator a ELISA Kit, MCL1, BCL2 family apoptosis regulator b ELISA Kit, small nucleolar RNA, H/ACA box 63 ELISA Kit, MCL1 ELISA Kit, Mcl1 ELISA Kit, mcl1a ELISA Kit, mcl1b ELISA Kit, SNORA63 ELISA Kit
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基因ID
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788087
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UniProt
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A5PJR2
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途径
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MAPK Pathway
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