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MCL-1 ELISA 试剂盒

MCL1 适用: Cow Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951150
发货至: 中国
  • 抗原 See all MCL-1 (MCL1) ELISA试剂盒
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    适用
    • 6
    • 3
    • 1
    • 1
    Cow
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Bovine MCL1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product MCL1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all MCL-1 (MCL1) ELISA试剂盒
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    别名
    MCL1 (MCL1 产品)
    别名
    BCL2L3 ELISA Kit, EAT ELISA Kit, MCL1-ES ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, Mcl-1 ELISA Kit, TM ELISA Kit, bcl2-L-3 ELISA Kit, mcl1/EAT ELISA Kit, AW556805 ELISA Kit, mcl-1 ELISA Kit, fb72f02 ELISA Kit, wu:fb70a08 ELISA Kit, wu:fb72f02 ELISA Kit, zfMLP1 ELISA Kit, zfMcl-1a ELISA Kit, zgc:109835 ELISA Kit, zgc:109763 ELISA Kit, E3 ELISA Kit, E3-2 ELISA Kit, RNE3 ELISA Kit, RNU107 ELISA Kit, SNORA63A ELISA Kit, elF-4AII ELISA Kit, MCL1, BCL2 family apoptosis regulator ELISA Kit, myeloid cell leukemia sequence 1 ELISA Kit, MCL1, BCL2 family apoptosis regulator a ELISA Kit, MCL1, BCL2 family apoptosis regulator b ELISA Kit, small nucleolar RNA, H/ACA box 63 ELISA Kit, MCL1 ELISA Kit, Mcl1 ELISA Kit, mcl1a ELISA Kit, mcl1b ELISA Kit, SNORA63 ELISA Kit
    基因ID
    788087
    UniProt
    A5PJR2
    途径
    MAPK Pathway
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