ING5 ELISA 试剂盒
ING5
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ING5 products
- ING5 (Inhibitor of Growth Family, Member 5 (ING5))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ING5 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all ING5 products
- ING5 (Inhibitor of Growth Family, Member 5 (ING5))
- 别名
- ING5 (ING5 产品)
- 别名
- p28ING5 ELISA Kit, 1700001C14Rik ELISA Kit, 1700027H23Rik ELISA Kit, 1810018M11Rik ELISA Kit, AI225768 ELISA Kit, p28ing5 ELISA Kit, MGC76007 ELISA Kit, ING5 ELISA Kit, MGC133862 ELISA Kit, ING1 ELISA Kit, p33 ELISA Kit, si:busm1-40g1.1 ELISA Kit, si:dz40g1.1 ELISA Kit, wu:fc94a02 ELISA Kit, zgc:100910 ELISA Kit, ing5 ELISA Kit, inhibitor of growth family member 5 ELISA Kit, inhibitor of growth family, member 5 ELISA Kit, inhibitor of growth family member 5 L homeolog ELISA Kit, inhibitor of growth family, member 5b ELISA Kit, inhibitor of growth family, member 5a ELISA Kit, ING5 ELISA Kit, Ing5 ELISA Kit, ing5 ELISA Kit, ing5.L ELISA Kit, ing5b ELISA Kit, ing5a ELISA Kit
- 基因ID
- 84289
- UniProt
- Q8WYH8
- 途径
- Chromatin Binding
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