PRDM2 ELISA 试剂盒
PRDM2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all PRDM2 products
- PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human PRDM2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all PRDM2 products
- PRDM2 (PR Domain Containing 2, with ZNF Domain (PRDM2))
- 别名
- PRDM2 (PRDM2 产品)
- 别名
- MGC114763 ELISA Kit, prdm2 ELISA Kit, riz ELISA Kit, kmt8 ELISA Kit, riz1 ELISA Kit, riz2 ELISA Kit, mtb-zf ELISA Kit, humhoxy1 ELISA Kit, PRDM2 ELISA Kit, HUMHOXY1 ELISA Kit, KMT8 ELISA Kit, MTB-ZF ELISA Kit, RIZ ELISA Kit, RIZ1 ELISA Kit, RIZ2 ELISA Kit, 4833427P12Rik ELISA Kit, E330024L24Rik ELISA Kit, Gm1033 ELISA Kit, Gm19732 ELISA Kit, Riz ELISA Kit, Riz1 ELISA Kit, Znfpr1c1 ELISA Kit, PR domain containing 2, with ZNF domain S homeolog ELISA Kit, PR domain containing 2, with ZNF domain b ELISA Kit, PR domain containing 2, with ZNF domain ELISA Kit, PR domain zinc finger protein 2 ELISA Kit, PR/SET domain 2 ELISA Kit, prdm2.S ELISA Kit, prdm2b ELISA Kit, prdm2 ELISA Kit, LOC100350859 ELISA Kit, PRDM2 ELISA Kit, Prdm2 ELISA Kit
- 基因ID
- 7799
- UniProt
- Q13029
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