FKBP1A ELISA 试剂盒
-
- 抗原 See all FKBP1A ELISA试剂盒
- FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human FKBP12 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product FKBP1A ELISA Kit
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all FKBP1A ELISA试剂盒
- FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))
- 别名
- FKBP12 (FKBP1A 产品)
- 别名
- 12kDa ELISA Kit, FKBP12 ELISA Kit, FKBP12-T1 ELISA Kit, FKBP12-T2 ELISA Kit, Fkbp ELISA Kit, Fkbp1 ELISA Kit, mFKBP1 ELISA Kit, mFKBP12 ELISA Kit, FKBP-12 ELISA Kit, FKBP-1A ELISA Kit, FKBP1 ELISA Kit, Rotamase ELISA Kit, Fkbp12 ELISA Kit, ARABIDOPSIS THALIANA FK506-BINDING PROTEIN 12 ELISA Kit, ATFKBP12 ELISA Kit, FK506-binding protein 12 ELISA Kit, IMMUNOPHILIN ELISA Kit, MSJ1.19 ELISA Kit, MSJ1_19 ELISA Kit, PKC12 ELISA Kit, PKCI2 ELISA Kit, PPIASE ELISA Kit, FKBP1B ELISA Kit, Fkbp2 ELISA Kit, fj02g10 ELISA Kit, zgc:73373 ELISA Kit, wu:fj02g10 ELISA Kit, FKBP1A ELISA Kit, Bmfkbp13 ELISA Kit, zgc:103752 ELISA Kit, FK506 binding protein 1a ELISA Kit, FK506 binding protein 1A ELISA Kit, FK506-binding protein ELISA Kit, FK506-binding protein 12 ELISA Kit, FK506 binding protein 1Aa ELISA Kit, FK506 binding protein 1C ELISA Kit, FK506-binding protein 59 ELISA Kit, FK506 binding protein like ELISA Kit, FK506 binding protein 1A L homeolog ELISA Kit, FK506 binding protein 1Ab ELISA Kit, Fkbp1a ELISA Kit, FKBP1A ELISA Kit, Fkbp12 ELISA Kit, FKBP12 ELISA Kit, fkbp1aa ELISA Kit, FKBP1C ELISA Kit, EHI_180160 ELISA Kit, LOC5573443 ELISA Kit, Fkbp13 ELISA Kit, FKBPL ELISA Kit, fkbp1a.L ELISA Kit, fkbp1ab ELISA Kit
- 基因ID
- 2280
- UniProt
- P62942
- 途径
- Negative Regulation of Transporter Activity, Methionine Biosynthetic Process
-