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Ephrin B3 ELISA 试剂盒

EFNB3 适用: 大鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951118
发货至: 中国
  • 抗原 See all Ephrin B3 (EFNB3) ELISA试剂盒
    Ephrin B3 (EFNB3)
    适用
    • 3
    • 1
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Rat Ephrin-B3 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product EFNB3 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Ephrin B3 (EFNB3) ELISA试剂盒
    Ephrin B3 (EFNB3)
    别名
    Ephrin-B3 (EFNB3 产品)
    别名
    efnb3-A ELISA Kit, EphB3 ELISA Kit, EFNB3 ELISA Kit, efnb3 ELISA Kit, id:ibd5049 ELISA Kit, zgc:111938 ELISA Kit, EFL-6 ELISA Kit, ELF-3 ELISA Kit, Elk-L3 ELISA Kit, Epl8 ELISA Kit, LERK-8 ELISA Kit, NLERK-2 ELISA Kit, ELK-L3 ELISA Kit, RGD1565678 ELISA Kit, EFL6 ELISA Kit, EPLG8 ELISA Kit, LERK8 ELISA Kit, ephrin B3 ELISA Kit, ephrin-B3 L homeolog ELISA Kit, ephrin-B3b ELISA Kit, ephrin-B3 ELISA Kit, EFNB3 ELISA Kit, efnb3.L ELISA Kit, efnb3b ELISA Kit, efnb3 ELISA Kit, Efnb3 ELISA Kit
    基因ID
    360546
    UniProt
    G3V7D4
    途径
    RTK signaling
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