Coagulation Factor IX ELISA 试剂盒
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- 抗原 See all Coagulation Factor IX (F9) ELISA试剂盒
- Coagulation Factor IX (F9)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human F9 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product F9 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all Coagulation Factor IX (F9) ELISA试剂盒
- Coagulation Factor IX (F9)
- 别名
- F9 (F9 产品)
- 别名
- AA407498 ELISA Kit, AI506714 ELISA Kit, AK-3 ELISA Kit, Ak3l ELISA Kit, Ak3l1 ELISA Kit, Akl3l ELISA Kit, FIX ELISA Kit, HEMB ELISA Kit, P19 ELISA Kit, PTC ELISA Kit, THPH8 ELISA Kit, AW111646 ELISA Kit, Cf-9 ELISA Kit, Cf9 ELISA Kit, f9 ELISA Kit, zgc:109775 ELISA Kit, fix ELISA Kit, ptc ELISA Kit, hemb ELISA Kit, FIXA ELISA Kit, zgc:136807 ELISA Kit, adenylate kinase 3 ELISA Kit, coagulation factor IX ELISA Kit, coagulation factor IXa ELISA Kit, coagulation factor 9 ELISA Kit, coagulation factor 9 S homeolog ELISA Kit, Coagulation factor IX ELISA Kit, coagulation factor IXb ELISA Kit, Ak3 ELISA Kit, F9 ELISA Kit, f9a ELISA Kit, f9 ELISA Kit, CpipJ_CPIJ003776 ELISA Kit, CpipJ_CPIJ009129 ELISA Kit, CpipJ_CPIJ013063 ELISA Kit, CpipJ_CPIJ013624 ELISA Kit, Tsp_01441 ELISA Kit, f9.S ELISA Kit, fa9 ELISA Kit, f9b ELISA Kit
- 基因ID
- 2158
- UniProt
- P00740
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