Casein Kinase 1 gamma 2 ELISA 试剂盒
CSNK1G2
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all Casein Kinase 1 gamma 2 (CSNK1G2) products
- Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CSNK1G2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all Casein Kinase 1 gamma 2 (CSNK1G2) products
- Casein Kinase 1 gamma 2 (CSNK1G2) (Casein Kinase 1, gamma 2 (CSNK1G2))
- 别名
- CSNK1G2 (CSNK1G2 产品)
- 别名
- CK1g2 ELISA Kit, 2810429I12Rik ELISA Kit, AI463719 ELISA Kit, CKI ELISA Kit, ck1-gamma ELISA Kit, CSNK1G2 ELISA Kit, ck1g2 ELISA Kit, csnk1g2 ELISA Kit, fj97g11 ELISA Kit, wu:fj97g11 ELISA Kit, wz8345 ELISA Kit, zgc:110719 ELISA Kit, zgc:153415 ELISA Kit, casein kinase 1 gamma 2 ELISA Kit, casein kinase 1, gamma 2 ELISA Kit, casein kinase 1 gamma 2 L homeolog ELISA Kit, casein kinase 1, gamma 2a ELISA Kit, casein kinase 1, gamma 2b ELISA Kit, CSNK1G2 ELISA Kit, Csnk1g2 ELISA Kit, csnk1g2.L ELISA Kit, csnk1g2 ELISA Kit, csnk1g2a ELISA Kit, csnk1g2b ELISA Kit
- 基因ID
- 1455
- UniProt
- P78368
- 途径
- Hedgehog Signaling
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