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CD6 ELISA 试剂盒

CD6 适用: 大鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951097
发货至: 中国
  • 抗原 See all CD6 ELISA试剂盒
    CD6
    适用
    • 5
    • 5
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Rat CD6 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CD6 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all CD6 ELISA试剂盒
    CD6
    别名
    CD6 (CD6 产品)
    别名
    TP120 ELISA Kit, OX52 ELISA Kit, CD6 antigen ELISA Kit, CD6 molecule ELISA Kit, Cd6 molecule ELISA Kit, Cd6 ELISA Kit, CD6 ELISA Kit
    基因ID
    25752
    UniProt
    G3V8U0
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