ATOX1 ELISA 试剂盒
ATOX1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ATOX1 products
- ATOX1 (ATX1 Antioxidant Protein 1 Homolog (ATOX1))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ATOX1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all ATOX1 products
- ATOX1 (ATX1 Antioxidant Protein 1 Homolog (ATOX1))
- 别名
- ATOX1 (ATOX1 产品)
- 别名
- ATX1 ELISA Kit, HAH1 ELISA Kit, AI256639 ELISA Kit, Atx1 ELISA Kit, CG12562 ELISA Kit, CG32446 ELISA Kit, DmAtox1 ELISA Kit, Dmel\\CG32446 ELISA Kit, atx1 ELISA Kit, hah1 ELISA Kit, DDBDRAFT_0185901 ELISA Kit, DDBDRAFT_0266724 ELISA Kit, DDB_0185901 ELISA Kit, DDB_0266724 ELISA Kit, SAH ELISA Kit, ATOX1 ELISA Kit, antioxidant 1 copper chaperone ELISA Kit, Antioxidant 1 copper chaperone ELISA Kit, copper transport protein ELISA Kit, ATX1 antioxidant protein 1 homolog ELISA Kit, ATOX1 ELISA Kit, Atox1 ELISA Kit, atox1 ELISA Kit
- 基因ID
- 475
- UniProt
- O00244
- 途径
- Transition Metal Ion Homeostasis
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