ALDH3A1 ELISA 试剂盒
ALDH3A1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ALDH3A1 ELISA试剂盒
- ALDH3A1 (Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ALDH3A1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ALDH3A1 ELISA Kit
-
Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1) ELISA Kit
ALDH3A1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all ALDH3A1 ELISA试剂盒
- ALDH3A1 (Aldehyde Dehydrogenase 3 Family, Member A1 (ALDH3A1))
- 别名
- ALDH3A1 (ALDH3A1 产品)
- 别名
- ALDC ELISA Kit, ALDH-E1 ELISA Kit, ALDH1 ELISA Kit, ALDH11 ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, ALDH3 ELISA Kit, ALDHIII ELISA Kit, Ahd-4 ELISA Kit, Ahd4 ELISA Kit, Aldh ELISA Kit, Aldh3 ELISA Kit, AHDC ELISA Kit, Ahd-c ELISA Kit, aldehyde dehydrogenase 1 family member A1 ELISA Kit, aldehyde dehydrogenase 3 family member A1 ELISA Kit, aldehyde dehydrogenase ELISA Kit, aldehyde dehydrogenase, dimeric NADP-preferring ELISA Kit, aldehyde dehydrogenase family 3, subfamily A1 ELISA Kit, aldehyde dehydrogenase 3 family, member A1 ELISA Kit, ALDH1A1 ELISA Kit, ALDH3A1 ELISA Kit, OE_RS03630 ELISA Kit, LOC100077088 ELISA Kit, CLL_A1665 ELISA Kit, LOC100281700 ELISA Kit, LOC100285809 ELISA Kit, Aldh3a1 ELISA Kit
- 基因ID
- 218
- UniProt
- P30838
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