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IGFBPL1 ELISA 试剂盒

IGFBPL1 适用: 小鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6810492
发货至: 中国
  • 抗原 See all IGFBPL1 products
    IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
    适用
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Mouse IGFBP-L1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Mouse IGFBP-L1.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all IGFBPL1 products
    IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))
    别名
    IGFBP-L1 (IGFBPL1 产品)
    别名
    IGFBP-RP4 ELISA Kit, IGFBPRP4 ELISA Kit, bA113O24.1 ELISA Kit, 2810011G06Rik ELISA Kit, 2810453O06Rik ELISA Kit, IGFBPL ELISA Kit, insulin like growth factor binding protein like 1 ELISA Kit, insulin-like growth factor binding protein-like 1 ELISA Kit, IGFBPL1 ELISA Kit, Igfbpl1 ELISA Kit
    UniProt
    Q80W15
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