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Hemoglobin Subunit beta ELISA 试剂盒

HBB 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6810489
发货至: 中国
  • 抗原 See all Hemoglobin Subunit beta (HBB) ELISA试剂盒
    Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human HbA1c/HBB ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human HbA1c.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product HBB ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Hemoglobin Subunit beta (HBB) ELISA试剂盒
    Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
    别名
    HBB (HBB 产品)
    别名
    HBB ELISA Kit, HBB1 ELISA Kit, HBBA ELISA Kit, HBBB ELISA Kit, CD113t-C ELISA Kit, beta-globin ELISA Kit, hbb ELISA Kit, hemoglobin, beta ELISA Kit, hemoglobin subunit beta ELISA Kit, hemoglobin subunit beta-like ELISA Kit, beta globin ELISA Kit, hemoglobin beta chain complex ELISA Kit, hemoglobin subunit beta-1 ELISA Kit, HBB ELISA Kit, HBB2 ELISA Kit, LOC100720944 ELISA Kit, LOC480784 ELISA Kit, Hbb ELISA Kit, LOC100136576 ELISA Kit, LOC101098159 ELISA Kit, LOC100976465 ELISA Kit, LOC100410611 ELISA Kit, LOC105468624 ELISA Kit
    物质类
    Viral Protein
    UniProt
    P68871
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