Sonic Hedgehog IQ-ELISA 试剂盒
-
- 抗原 See all Sonic Hedgehog (SHH) IQ-ELISA Kits
- Sonic Hedgehog (SHH)
-
适用
- 人
- 检测方法
- qPCR
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- Human Immunoquantitative (PCR-Based) Sonic Hedgehog N-Terminal ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- 4.2 pg/ml in Assay Diluent C, and8 pg/ml in Assay Diluent B. (Anticipated minimum sensitivity. Assay may detect lower levels of antigen.)
- 灵敏度
- 4.2 pg/mL
- 产品特性
- The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human Sonic Hedgehog N-Terminal IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Sonic Hedgehog N-Terminal in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Sonic Hedgehog N-Terminal coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Sonic Hedgehog N-Terminal present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
- 组件
-
- Sonic Hedgehog N-Terminal Microplate (Item A): 96 well PCR plate coated with anti-Human Sonic Hedgehog N-Terminal
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human Sonic Hedgehog N-Terminal
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for Sonic Hedgehog N-Terminal (Item F): 2 vials of a 4x concentrated solution of anti-Human Sonic Hedgehog N-Terminal affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- 试剂未包括
-
- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Featured
- Discover our best selling SHH ELISA Kit
- Top Product
- Discover our top product SHH ELISA Kit
-
Sonic Hedgehog (SHH) ELISA Kit
SHH 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 小鼠 Colorimetric Sandwich ELISA 28 pg/mL - 1800 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sonic Hedgehog (SHH) ELISA KitSmall Sample SHH 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 小鼠 Colorimetric Sandwich ELISA 5-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Sonic Hedgehog (SHH) ELISA KitSHH 适用: 小鼠 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
-
- 应用备注
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- 说明
-
Features:
- Excellent for follow up/confirmation of antibody array data
- Pre-coated 96 well plate
- Strip plate format minimizes waste
- Complete kit includes all necessary reagents
- 10x more sensitive than ELISA
- Reproducible and reliable
- Optimized for serum, plasma, urine and cell culture medium
- 1/10th the sample volume of an ELISA
Research Applications:
- Detection of quantitative protein levels in biological fluids
- Validation of antibody array results
- Validation of biomarker discovery studies - 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- 限制
- 仅限研究用
-
- 储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.
- 有效期
- 6 months
-
- 抗原 See all Sonic Hedgehog (SHH) IQ-ELISA Kits
- Sonic Hedgehog (SHH)
- 别名
- Sonic Hedgehog (SHH 产品)
- 别名
- HHG1 IQ-ELISA Kit, HLP3 IQ-ELISA Kit, HPE3 IQ-ELISA Kit, MCOPCB5 IQ-ELISA Kit, SMMCI IQ-ELISA Kit, TPT IQ-ELISA Kit, TPTPS IQ-ELISA Kit, 9530036O11Rik IQ-ELISA Kit, Dsh IQ-ELISA Kit, Hhg1 IQ-ELISA Kit, Hx IQ-ELISA Kit, Hxl3 IQ-ELISA Kit, M100081 IQ-ELISA Kit, fc83d08 IQ-ELISA Kit, shh IQ-ELISA Kit, syu IQ-ELISA Kit, vhh-1 IQ-ELISA Kit, vhh1 IQ-ELISA Kit, wu:fc83d08 IQ-ELISA Kit, Xhh IQ-ELISA Kit, hedgehog IQ-ELISA Kit, xshh IQ-ELISA Kit, SHH IQ-ELISA Kit, twh IQ-ELISA Kit, twhh IQ-ELISA Kit, sonic hedgehog IQ-ELISA Kit, sonic hedgehog a IQ-ELISA Kit, sonic hedgehog L homeolog IQ-ELISA Kit, sonic hedgehog protein A IQ-ELISA Kit, sonic hedgehog b IQ-ELISA Kit, SHH IQ-ELISA Kit, Shh IQ-ELISA Kit, shha IQ-ELISA Kit, shh.L IQ-ELISA Kit, shh IQ-ELISA Kit, shhb IQ-ELISA Kit
- 基因ID
- 6469
- UniProt
- Q15465
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
-
