NPM1 ELISA 试剂盒
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- 抗原 See all NPM1 ELISA试剂盒
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human NPM1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human NPM1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product NPM1 ELISA Kit
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Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) ELISA Kit
NPM1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) ELISA KitNPM1 适用: 人 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all NPM1 ELISA试剂盒
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
- 别名
- NPM1 (NPM1 产品)
- 别名
- B23 ELISA Kit, NPM ELISA Kit, NO38 ELISA Kit, Npm ELISA Kit, B23NP ELISA Kit, npm4 ELISA Kit, npm ELISA Kit, CG7917 ELISA Kit, CRP1 ELISA Kit, Crp1 ELISA Kit, Dmel\\CG7917 ELISA Kit, dNLP ELISA Kit, dNLP-S ELISA Kit, p22 ELISA Kit, npm1 ELISA Kit, sb:cb487 ELISA Kit, nucleophosmin 1 ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog ELISA Kit, nucleophosmin 1b ELISA Kit, Nucleophosmin ELISA Kit, nucleophosmin ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit, Nucleoplasmin ELISA Kit, nucleophosmin 1a ELISA Kit, NPM1 ELISA Kit, Npm1 ELISA Kit, npm1.L ELISA Kit, npm1b ELISA Kit, npm ELISA Kit, Nlp ELISA Kit, npm1a ELISA Kit
- UniProt
- P06748
- 途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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