EPH Receptor A7 ELISA 试剂盒
EPHA7
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all EPH Receptor A7 (EPHA7) ELISA试剂盒
- EPH Receptor A7 (EPHA7)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse EphA7 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse EphA7.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product EPHA7 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all EPH Receptor A7 (EPHA7) ELISA试剂盒
- EPH Receptor A7 (EPHA7)
- 别名
- EphA7 (EPHA7 产品)
- 别名
- fc51g03 ELISA Kit, wu:fc51g03 ELISA Kit, si:dkey-23k6.1 ELISA Kit, EHK3 ELISA Kit, HEK11 ELISA Kit, Cek11 ELISA Kit, Ebk ELISA Kit, Ehk3 ELISA Kit, Hek11 ELISA Kit, Mdk1 ELISA Kit, CEPHA7 ELISA Kit, EPH receptor A7 ELISA Kit, eph receptor A7 ELISA Kit, ephrin type-B receptor 1-B ELISA Kit, Eph receptor A7 ELISA Kit, EPHA7 ELISA Kit, epha7 ELISA Kit, LOC100166161 ELISA Kit, Epha7 ELISA Kit
- UniProt
- Q61772
- 途径
- RTK signaling
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