Prothrombin ELISA 试剂盒
-
- 抗原 See all Prothrombin (F2) ELISA试剂盒
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Coagulation Factor II/F2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Coagulation Factor II.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product F2 ELISA Kit
-
Coagulation Factor II (thrombin) (F2) ELISA Kit
F2 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Colorimetric Sandwich ELISA 9.38 ng/mL - 600 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Colorimetric Sandwich ELISA 0.41 ng/mL - 300 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: Cow Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Serum
Coagulation Factor II (thrombin) (F2) ELISA KitF2 适用: 人 Fragment 1 Colorimetric Sandwich ELISA 0.03-2 nM/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all Prothrombin (F2) ELISA试剂盒
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- 别名
- Coagulation Factor II (F2 (F2 产品)
- 别名
- PT ELISA Kit, RPRGL2 ELISA Kit, THPH1 ELISA Kit, Cf-2 ELISA Kit, Cf2 ELISA Kit, FII ELISA Kit, thrombin ELISA Kit, zgc:66319 ELISA Kit, wu:fb57c10 ELISA Kit, wu:fd42e09 ELISA Kit, wu:fd59d01 ELISA Kit, prothrombin ELISA Kit, F2 ELISA Kit, coagulation factor II, thrombin ELISA Kit, coagulation factor II ELISA Kit, coagulation factor II (thrombin) ELISA Kit, F2 ELISA Kit, f2 ELISA Kit
- UniProt
- P19221
- 途径
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
-
