EPH Receptor A4 ELISA 试剂盒
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- 抗原 See all EPH Receptor A4 (EPHA4) ELISA试剂盒
- EPH Receptor A4 (EPHA4)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse EphA4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse EphA4.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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EPH Receptor A4 (EPHA4) ELISA Kit
EPHA4 适用: 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
EPH Receptor A4 (EPHA4) ELISA KitEPHA4 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
EPH Receptor A4 (EPHA4) ELISA KitEPHA4 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all EPH Receptor A4 (EPHA4) ELISA试剂盒
- EPH Receptor A4 (EPHA4)
- 别名
- EphA4 (EPHA4 产品)
- 别名
- HEK8 ELISA Kit, SEK ELISA Kit, TYRO1 ELISA Kit, RGD1560587 ELISA Kit, ephe ELISA Kit, EPHA4 ELISA Kit, Ci-Eph1 ELISA Kit, sek ELISA Kit, hek8 ELISA Kit, rtk1 ELISA Kit, tyr01 ELISA Kit, 2900005C20Rik ELISA Kit, AI385584 ELISA Kit, Cek8 ELISA Kit, Hek8 ELISA Kit, Sek ELISA Kit, Sek1 ELISA Kit, Tyro1 ELISA Kit, rb ELISA Kit, XEpha4 ELISA Kit, sek-1 ELISA Kit, epha4 ELISA Kit, pag ELISA Kit, EphA4 ELISA Kit, apha3 ELISA Kit, ek2 ELISA Kit, epha3 ELISA Kit, sb:cb246 ELISA Kit, zek2 ELISA Kit, zgc:109882 ELISA Kit, EPH receptor A4 ELISA Kit, Eph receptor A4 ELISA Kit, ephrin receptor epsilon ELISA Kit, EPH receptor A4 S homeolog ELISA Kit, EPH receptor A4 L homeolog ELISA Kit, eph receptor A4a ELISA Kit, EPHA4 ELISA Kit, Epha4 ELISA Kit, ephe ELISA Kit, epha4 ELISA Kit, epha4.S ELISA Kit, epha4.L ELISA Kit, epha4a ELISA Kit
- UniProt
- Q03137
- 途径
- RTK signaling
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