HGFA ELISA 试剂盒
-
- 抗原 See all HGFA ELISA试剂盒
- HGFA (Hepatocyte Growth Factor Activator (HGFA))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.045 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.045 ng/mL
- 应用范围
- ELISA
- 原理
- Human HGF Activator/HGFAC ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human HGF Activator.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product HGFA ELISA Kit
-
Hepatocyte Growth Factor Activator (HGFA) ELISA Kit
HGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Hepatocyte Growth Factor Activator (HGFA) ELISA KitHGFA 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Hepatocyte Growth Factor Activator (HGFA) ELISA KitHGFA 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Hepatocyte Growth Factor Activator (HGFA) ELISA KitHGFA 适用: 人 AA 36-655 Colorimetric Sandwich ELISA
Hepatocyte Growth Factor Activator (HGFA) ELISA KitHGFA 适用: 人 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
-
Recommended Dilution for serum and plasma samples2000 fold
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all HGFA ELISA试剂盒
- HGFA (Hepatocyte Growth Factor Activator (HGFA))
- 别名
- HGF Activator (HGFAC (HGFA 产品)
- 别名
- HGFA ELISA Kit, HGF activator ELISA Kit, hepatocyte growth factor a ELISA Kit, hepatocyte growth factor activator ELISA Kit, HGFAC ELISA Kit, hgfa ELISA Kit, LOC100303670 ELISA Kit, Hgfac ELISA Kit
- UniProt
- Q04756
-
