KIRREL2 ELISA 试剂盒
KIRREL2
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
- 抗原 See all KIRREL2 products
- KIRREL2 (Kin of IRRE Like 2 (Drosophila) (KIRREL2))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Kirrel2/NEPH3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Kirrel2.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all KIRREL2 products
- KIRREL2 (Kin of IRRE Like 2 (Drosophila) (KIRREL2))
- 别名
- Kirrel2 (NEPH3 (KIRREL2 产品)
- 别名
- nlg1 ELISA Kit, neph3 ELISA Kit, filtrin ELISA Kit, FILTRIN ELISA Kit, NEPH3 ELISA Kit, NLG1 ELISA Kit, C330019F22Rik ELISA Kit, cb33 ELISA Kit, kirrel3 ELISA Kit, sb:cb33 ELISA Kit, si:dkey-29g23.1 ELISA Kit, wu:fb74e02 ELISA Kit, kirre like nephrin family adhesion molecule 2 ELISA Kit, kin of IRRE like 2 (Drosophila) ELISA Kit, kirre like nephrin family adhesion molecule 3, like ELISA Kit, kirrel2 ELISA Kit, KIRREL2 ELISA Kit, Kirrel2 ELISA Kit, kirrel3l ELISA Kit
- UniProt
- Q7TSU7
-
You are here:
