EPH Receptor A2 ELISA 试剂盒
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- 抗原 See all EPH Receptor A2 (EPHA2) ELISA试剂盒
- EPH Receptor A2 (EPHA2)
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse EphA2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse EphA2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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EPH Receptor A2 (EPHA2) ELISA Kit
EPHA2 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
EPH Receptor A2 (EPHA2) ELISA KitEPHA2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all EPH Receptor A2 (EPHA2) ELISA试剂盒
- EPH Receptor A2 (EPHA2)
- 别名
- EphA2 (EPHA2 产品)
- 别名
- ARCC2 ELISA Kit, CTPA ELISA Kit, CTPP1 ELISA Kit, CTRCT6 ELISA Kit, ECK ELISA Kit, epha2 ELISA Kit, MGC89505 ELISA Kit, AW545284 ELISA Kit, Eck ELISA Kit, Myk2 ELISA Kit, Sek-2 ELISA Kit, Sek2 ELISA Kit, id:ibd5152 ELISA Kit, rtk6 ELISA Kit, G42 ELISA Kit, XE10 ELISA Kit, epha2-A ELISA Kit, EPH receptor A2 ELISA Kit, Eph receptor A2 ELISA Kit, epoxide hydrolase ELISA Kit, eph receptor A2 a ELISA Kit, EPH receptor A2 L homeolog ELISA Kit, EPHA2 ELISA Kit, Epha2 ELISA Kit, epha2 ELISA Kit, ephA2 ELISA Kit, epha2a ELISA Kit, epha2.L ELISA Kit
- UniProt
- Q03145
- 途径
- RTK signaling
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