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ABL1 ELISA 试剂盒

ABL1 适用: 人 pTyr245 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
产品编号 ABIN6730567
发货至: 中国
  • 抗原 See all ABL1 ELISA试剂盒
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    抗原表位
    pTyr245
    适用
    • 6
    • 4
    • 4
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Phospho-c-Abl (Tyr245) ELISA Kit. This assay semi-quantitatively measures c-Abl phosphorylated at Tyrosine-245 in cell lysate samples.
    样品类型
    Cell Samples, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    特异性
    This ELISA kit recognizes Human c-Abl phosphorylated at site Tyrosine-245.
    产品特性
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    试剂未包括
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling ABL1 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    有效期
    6 months
  • 抗原 See all ABL1 ELISA试剂盒
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    别名
    c-Abl (ABL1 产品)
    别名
    ABL ELISA Kit, JTK7 ELISA Kit, bcr/abl ELISA Kit, c-ABL ELISA Kit, p150 ELISA Kit, v-abl ELISA Kit, AI325092 ELISA Kit, Abl ELISA Kit, E430008G22Rik ELISA Kit, c-Abl ELISA Kit, ABL proto-oncogene 1, non-receptor tyrosine kinase ELISA Kit, c-abl oncogene 1, non-receptor tyrosine kinase ELISA Kit, Tyrosine-protein kinase abl-1 ELISA Kit, ABL1 ELISA Kit, Abl1 ELISA Kit, abl-1 ELISA Kit, abl1 ELISA Kit
    基因ID
    25
    UniProt
    P00519
    途径
    Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
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