ABL1 ELISA 试剂盒
ABL1
适用: 人
pTyr245
Colorimetric
Sandwich ELISA
Cell Samples, Tissue Lysate
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- 抗原 See all ABL1 ELISA试剂盒
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
- 抗原表位
- pTyr245
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phospho-c-Abl (Tyr245) ELISA Kit. This assay semi-quantitatively measures c-Abl phosphorylated at Tyrosine-245 in cell lysate samples.
- 样品类型
- Cell Samples, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes Human c-Abl phosphorylated at site Tyrosine-245.
- 产品特性
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling ABL1 ELISA Kit
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C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all ABL1 ELISA试剂盒
- ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
- 别名
- c-Abl (ABL1 产品)
- 别名
- ABL ELISA Kit, JTK7 ELISA Kit, bcr/abl ELISA Kit, c-ABL ELISA Kit, p150 ELISA Kit, v-abl ELISA Kit, AI325092 ELISA Kit, Abl ELISA Kit, E430008G22Rik ELISA Kit, c-Abl ELISA Kit, ABL proto-oncogene 1, non-receptor tyrosine kinase ELISA Kit, c-abl oncogene 1, non-receptor tyrosine kinase ELISA Kit, Tyrosine-protein kinase abl-1 ELISA Kit, ABL1 ELISA Kit, Abl1 ELISA Kit, abl-1 ELISA Kit, abl1 ELISA Kit
- 基因ID
- 25
- UniProt
- P00519
- 途径
- Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
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