ADAM15 ELISA 试剂盒
ADAM15
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all ADAM15 ELISA试剂盒
- ADAM15 (ADAM Metallopeptidase Domain 15 (ADAM15))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ADAM15 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human ADAM15.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ADAM15 ELISA Kit
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ADAM Metallopeptidase Domain 15 (ADAM15) ELISA Kit
ADAM15 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase Domain 15 (ADAM15) ELISA KitADAM15 适用: 人 AA 207-696 Colorimetric Sandwich ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all ADAM15 ELISA试剂盒
- ADAM15 (ADAM Metallopeptidase Domain 15 (ADAM15))
- 别名
- ADAM15 (ADAM15 产品)
- 别名
- ADAM15 ELISA Kit, mdc15 ELISA Kit, MDC15 ELISA Kit, metargidin ELISA Kit, tMDCVI ELISA Kit, ADAM metallopeptidase domain 15 ELISA Kit, ADAM metallopeptidase domain 15 L homeolog ELISA Kit, a disintegrin and metallopeptidase domain 15 (metargidin) ELISA Kit, ADAM15 ELISA Kit, adam15.L ELISA Kit, adam15 ELISA Kit, Adam15 ELISA Kit
- UniProt
- Q13444
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