YWHAE ELISA 试剂盒
YWHAE
适用: 人
Colorimetric
Sandwich ELISA
0.34 ng/mL - 80 ng/mL
Cell Culture Supernatant, Plasma, Serum
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- 抗原 See all YWHAE ELISA试剂盒
- YWHAE (14-3-3 epsilon (YWHAE))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.34 ng/mL - 80 ng/mL
- 最低检测浓度
- 0.34 ng/mL
- 应用范围
- ELISA
- 原理
- Human YWHAE ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human YWHAE.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product YWHAE ELISA Kit
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14-3-3 epsilon (YWHAE) ELISA Kit
YWHAE 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
14-3-3 epsilon (YWHAE) ELISA KitYWHAE 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 试剂准备
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Recommended Dilution for serum and plasma samples2 fold
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all YWHAE ELISA试剂盒
- YWHAE (14-3-3 epsilon (YWHAE))
- 别名
- YWHAE (YWHAE 产品)
- 别名
- 14-3-3 ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3-e ELISA Kit, 14-3-3-epsilon ELISA Kit, 14-3-30epsilon ELISA Kit, 14-3-3EPSILON ELISA Kit, 14-3-3e ELISA Kit, 14-3-3omicron ELISA Kit, 1433epsilon ELISA Kit, 143E_DROME ELISA Kit, CG31196 ELISA Kit, CT24092 ELISA Kit, D14-3-3e ELISA Kit, D14-3-3epsilon ELISA Kit, Dmel\\CG31196 ELISA Kit, EK3-5 ELISA Kit, PAR-5 ELISA Kit, PAR5 ELISA Kit, Par-5 ELISA Kit, SR3-9 ELISA Kit, Su(Raf)3B ELISA Kit, anon-WO0172774.141 ELISA Kit, anon-WO02059370.52 ELISA Kit, d14-3-3epsilon ELISA Kit, eps ELISA Kit, l(3)j2B10 ELISA Kit, par-5 ELISA Kit, 14-3-3epsilon ELISA Kit, kcip-1 ELISA Kit, mdcr ELISA Kit, mds ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, ACYPI002517 ELISA Kit, YWHAE ELISA Kit, 14-3-3E ELISA Kit, KCIP-1 ELISA Kit, MDCR ELISA Kit, MDS ELISA Kit, AU019196 ELISA Kit, fe38c12 ELISA Kit, wu:fb48f06 ELISA Kit, wu:fe38c12 ELISA Kit, wu:fi22e10 ELISA Kit, ywhae ELISA Kit, zgc:55377 ELISA Kit, zgc:77062 ELISA Kit, CG31196 gene product from transcript CG31196-RD ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon ELISA Kit, 14-3-3 epsilon ELISA Kit, 14-3-3 epsilon protein ELISA Kit, 14-3-3 protein epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 ELISA Kit, 14-3-3epsilon ELISA Kit, ywhae ELISA Kit, LOC581376 ELISA Kit, Ywhae ELISA Kit, HCAG_04173 ELISA Kit, PTRG_02836 ELISA Kit, PTRG_04459 ELISA Kit, UREG_07864 ELISA Kit, YWHAE ELISA Kit, ywhae.S ELISA Kit, ywhae1 ELISA Kit
- UniProt
- P62258
- 途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
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