CTSC ELISA 试剂盒
CTSC
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CTSC ELISA试剂盒
- CTSC (Cathepsin C (CTSC))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Cathepsin C/DPPI ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Cathepsin C.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CTSC ELISA Kit
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Cathepsin C (CTSC) ELISA Kit
CTSC 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Cathepsin C (CTSC) ELISA KitCTSC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CTSC ELISA试剂盒
- CTSC (Cathepsin C (CTSC))
- 别名
- Cathepsin C (DPPI (CTSC 产品)
- 别名
- CPPI ELISA Kit, DPP-I ELISA Kit, DPP1 ELISA Kit, DPPI ELISA Kit, HMS ELISA Kit, JP ELISA Kit, JPD ELISA Kit, PALS ELISA Kit, PDON1 ELISA Kit, PLS ELISA Kit, MGC69126 ELISA Kit, MGC107782 ELISA Kit, AI047818 ELISA Kit, CATC ELISA Kit, cb912 ELISA Kit, ik:tdsubc_1h2 ELISA Kit, sb:cb146 ELISA Kit, wu:fb34g12 ELISA Kit, wu:fj58d01 ELISA Kit, cathepsin C ELISA Kit, cathepsin C L homeolog ELISA Kit, cathepsin CPC1 ELISA Kit, CTSC ELISA Kit, ctsc.L ELISA Kit, ctsc ELISA Kit, TP03_0357 ELISA Kit, CMU_003360 ELISA Kit, CPC1 ELISA Kit, NAEGRDRAFT_80477 ELISA Kit, NAEGRDRAFT_37577 ELISA Kit, Ctsc ELISA Kit, Smp_183330 ELISA Kit
- UniProt
- P97821
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