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TRH ELISA 试剂盒

TRH 适用: 大鼠, 人, 小鼠 Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
产品编号 ABIN6730541
发货至: 中国
  • 抗原 See all TRH ELISA试剂盒
    TRH (Thyrotropin-Releasing Hormone (TRH))
    适用
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    大鼠, 人, 小鼠
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    检测范围
    0.1 ng/mL - 1000 ng/mL
    最低检测浓度
    0.1 ng/mL
    应用范围
    ELISA
    原理
    Human/Mouse/Rat Thyrotropin-releasing hormone EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
    样品类型
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This EIA kit is designed to detect human, mouse, and rat Thyrotropin-releasing hormone
    灵敏度
    0.6 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    试剂准备

    Recommended Dilution for serum and plasma samplesHuman: 8x / Mouse: 8x / Rat: 8x

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    有效期
    6 months
  • 抗原 See all TRH ELISA试剂盒
    TRH (Thyrotropin-Releasing Hormone (TRH))
    别名
    Thyrotropin-releasing hormone (TRH 产品)
    别名
    Pro-TRH ELISA Kit, TRF ELISA Kit, THR ELISA Kit, TRH01 ELISA Kit, Trf ELISA Kit, zgc:110168 ELISA Kit, TRH ELISA Kit, Pro-TRH-B ELISA Kit, trhp-a ELISA Kit, Pro-TRH-A ELISA Kit, trh ELISA Kit, thyrotropin releasing hormone ELISA Kit, thyrotropin-releasing hormone ELISA Kit, thyrotropin-releasing hormone S homeolog ELISA Kit, thyrotropin-releasing hormone L homeolog ELISA Kit, TRH ELISA Kit, Trh ELISA Kit, trh ELISA Kit, trh.S ELISA Kit, trh.L ELISA Kit
    基因ID
    7200
    UniProt
    P20396
    途径
    Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour
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