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JNK ELISA 试剂盒

MAPK8 适用: 小鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6730536
发货至: 中国
  • 抗原 See all JNK (MAPK8) ELISA试剂盒
    JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
    适用
    • 7
    • 6
    • 6
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Mouse JNK1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Mouse JNK1.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all JNK (MAPK8) ELISA试剂盒
    JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
    别名
    JNK1 (MAPK8 产品)
    别名
    JNK ELISA Kit, JNK-46 ELISA Kit, JNK1 ELISA Kit, JNK1A2 ELISA Kit, JNK21B1/2 ELISA Kit, PRKM8 ELISA Kit, SAPK1 ELISA Kit, SAPK1c ELISA Kit, AI849689 ELISA Kit, Prkm8 ELISA Kit, BSK ELISA Kit, BSK/DJNK ELISA Kit, Bsk ELISA Kit, CG5680 ELISA Kit, D-JNK ELISA Kit, D-junk ELISA Kit, DBSK/JNK ELISA Kit, DJNK ELISA Kit, DJNK/bsk ELISA Kit, Dmel\\CG5680 ELISA Kit, JNK/SAPK ELISA Kit, Jnk ELISA Kit, Junk ELISA Kit, SAPKa ELISA Kit, c-Jun ELISA Kit, dJNK ELISA Kit, jnk ELISA Kit, T10F20.15 ELISA Kit, jnk1 ELISA Kit, sapk1 ELISA Kit, mapk8 ELISA Kit, zgc:112379 ELISA Kit, JNKb ELISA Kit, mapk8b ELISA Kit, mitogen-activated protein kinase 8 ELISA Kit, Stress-activated protein kinase jnk-1 ELISA Kit, basket ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 8 L homeolog ELISA Kit, mitogen-activated protein kinase 8b ELISA Kit, mitogen-activated protein kinase 8B-like ELISA Kit, MAPK8 ELISA Kit, jnk-1 ELISA Kit, Mapk8 ELISA Kit, bsk ELISA Kit, ATMPK8 ELISA Kit, mapk8.L ELISA Kit, mapk8b ELISA Kit, LOC109094263 ELISA Kit
    UniProt
    A3KBF5
    途径
    MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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