JNK ELISA 试剂盒
MAPK8
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all JNK (MAPK8) ELISA试剂盒
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse JNK1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse JNK1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA Kit
MAPK8 适用: 人, 小鼠, 大鼠 pThr183 Colorimetric Cell ELISA Cell Culture Cells
Mitogen-Activated Protein Kinase 8 (MAPK8) ELISA KitMAPK8 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all JNK (MAPK8) ELISA试剂盒
- JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))
- 别名
- JNK1 (MAPK8 产品)
- 别名
- JNK ELISA Kit, JNK-46 ELISA Kit, JNK1 ELISA Kit, JNK1A2 ELISA Kit, JNK21B1/2 ELISA Kit, PRKM8 ELISA Kit, SAPK1 ELISA Kit, SAPK1c ELISA Kit, AI849689 ELISA Kit, Prkm8 ELISA Kit, BSK ELISA Kit, BSK/DJNK ELISA Kit, Bsk ELISA Kit, CG5680 ELISA Kit, D-JNK ELISA Kit, D-junk ELISA Kit, DBSK/JNK ELISA Kit, DJNK ELISA Kit, DJNK/bsk ELISA Kit, Dmel\\CG5680 ELISA Kit, JNK/SAPK ELISA Kit, Jnk ELISA Kit, Junk ELISA Kit, SAPKa ELISA Kit, c-Jun ELISA Kit, dJNK ELISA Kit, jnk ELISA Kit, T10F20.15 ELISA Kit, jnk1 ELISA Kit, sapk1 ELISA Kit, mapk8 ELISA Kit, zgc:112379 ELISA Kit, JNKb ELISA Kit, mapk8b ELISA Kit, mitogen-activated protein kinase 8 ELISA Kit, Stress-activated protein kinase jnk-1 ELISA Kit, basket ELISA Kit, Protein kinase superfamily protein ELISA Kit, mitogen-activated protein kinase 8 L homeolog ELISA Kit, mitogen-activated protein kinase 8b ELISA Kit, mitogen-activated protein kinase 8B-like ELISA Kit, MAPK8 ELISA Kit, jnk-1 ELISA Kit, Mapk8 ELISA Kit, bsk ELISA Kit, ATMPK8 ELISA Kit, mapk8.L ELISA Kit, mapk8b ELISA Kit, LOC109094263 ELISA Kit
- UniProt
- A3KBF5
- 途径
- MAPK Pathway, WNT signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins
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