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TPT1 ELISA 试剂盒
TPT1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
TPT1ELISA Kit详情
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TPT1
(Tumor Protein, Translationally-Controlled 1 (TPT1))
适用
All reactivities for TPT1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Human TPT1 ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human TPT1.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
TPT1目标详情
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TPT1
(Tumor Protein, Translationally-Controlled 1 (TPT1))
别名
TPT1 (TPT1 产品 )
别名
HRF ELISA Kit, TCTP ELISA Kit, p02 ELISA Kit, Trt ELISA Kit, p21 ELISA Kit, p23 ELISA Kit, TPT1 ELISA Kit, TCT1 ELISA Kit, tctp ELISA Kit, fa28a02 ELISA Kit, id:ibd2078 ELISA Kit, wu:fa28a02 ELISA Kit, PCHK23 ELISA Kit, hrf ELISA Kit, MGC79720 ELISA Kit, Tpt1 ELISA Kit, translationally controlled tumor protein ELISA Kit, CG4800 ELISA Kit, Dmel\\CG4800 ELISA Kit, dTCTP ELISA Kit, Tctp ELISA Kit, tumor protein, translationally-controlled 1 ELISA Kit, tumor protein, translationally-controlled 1 L homeolog ELISA Kit, translationally-controlled tumor protein ELISA Kit, translationally controlled tumor protein ELISA Kit, translationally controlled tumor-like protein ELISA Kit, Translationally controlled tumor protein ELISA Kit, translationally-controlled tumor protein homolog ELISA Kit, P23 tumor protein-like ELISA Kit, TPT1 ELISA Kit, Tpt1 ELISA Kit, tpt1 ELISA Kit, tpt1.L ELISA Kit, LOC711798 ELISA Kit, TCTP ELISA Kit, LOC548038 ELISA Kit, Tctp ELISA Kit, LOC108708523 ELISA Kit, LOC109404863 ELISA Kit, LOC105389397 ELISA Kit, LOC102577554 ELISA Kit
UniProt
P13693
途径
Response to Water Deprivation , Regulation of Cell Size , Negative Regulation of intrinsic apoptotic Signaling
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