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CRELD2 ELISA 试剂盒

CRELD2 适用: 小鼠 Colorimetric Sandwich ELISA 0.045 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN6730531
发货至: 中国
  • 抗原 See all CRELD2 ELISA试剂盒
    CRELD2 (Cysteine-Rich with EGF-Like Domains 2 (CRELD2))
    适用
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.045 ng/mL - 10 ng/mL
    最低检测浓度
    0.045 ng/mL
    应用范围
    ELISA
    原理
    Mouse CRELD2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Mouse CRELD2.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    试剂准备

    Recommended Dilution for serum and plasma samples50 fold

    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all CRELD2 ELISA试剂盒
    CRELD2 (Cysteine-Rich with EGF-Like Domains 2 (CRELD2))
    别名
    CRELD2 (CRELD2 产品)
    别名
    zgc:66383 ELISA Kit, CRELD2 ELISA Kit, 5730592L21Rik ELISA Kit, C85758 ELISA Kit, RGD1310614 ELISA Kit, cysteine-rich with EGF-like domains 2 ELISA Kit, cysteine rich with EGF like domains 2 ELISA Kit, creld2 ELISA Kit, CRELD2 ELISA Kit, Creld2 ELISA Kit
    UniProt
    Q9CYA0
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