PIN1 ELISA 试剂盒
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- 抗原 See all PIN1 ELISA试剂盒
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Pin1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human Pin1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PIN1 ELISA Kit
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- Discover our top product PIN1 ELISA Kit
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Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) ELISA Kit
PIN1 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) ELISA KitPIN1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) ELISA KitPIN1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PIN1 ELISA试剂盒
- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
- 别名
- Pin1 (PIN1 产品)
- 别名
- DOD ELISA Kit, UBL5 ELISA Kit, dod ELISA Kit, ubl5 ELISA Kit, pin1 ELISA Kit, pin1a ELISA Kit, 0610025L01Rik ELISA Kit, D9Bwg1161e ELISA Kit, pin1b ELISA Kit, zgc:73206 ELISA Kit, PPIase pin1 ELISA Kit, MdPin1 ELISA Kit, Pin1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog ELISA Kit, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog ELISA Kit, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 ELISA Kit, peptidyl-prolyl cis-trans isomerase Pin1 ELISA Kit, PIN1 ELISA Kit, Pin1 ELISA Kit, pin1 ELISA Kit, pin1.S ELISA Kit, pin1.L ELISA Kit, ppiD ELISA Kit, PITG_11123 ELISA Kit, LOC103419188 ELISA Kit
- UniProt
- Q13526
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